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Jun 09, 2020 · Cover the pressure cooker with the lid and let it boil on high flame. After 30 minutes or so, turn off the flame and let it cool before you release the pressure and remove the lid. Once your jars are cooled, now you can inoculate the substrate with the spores. Take the spore syringe with the needle and heat the needle with the lighter. Pressure cooker Your chosen plant (cauliflower, rose, African violet or carnation) paper towel for cutting on or sterile petri dishes if available Beaker or jar in which to wash the plant material Detergent-water mixture - 1ml detergent per liter of water Bleach sterilizing solution - dilute commercial bleach (5-6% sodium

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